Temporal changes in gene expression in the liver of male plaice (Pleuronectes platessa) in response to exposure to ethynyl oestradiol analysed by macroarray and Real-Time PCR.
作者： Margaret Brown , Craig Robinson , Ian M Davies , Colin F Moffat , John Redshaw
DOI: 10.1016/J.MRFMMM.2004.06.002
关键词:
摘要: Suppression subtractive hybridisation (SSH) was used to generate cDNA libraries representing genes differentially-expressed in liver from male plaice (Pleuronectes platessa) exposed ethynyl oestradiol (EE2). BLAST analysis and alignments of the clones with database sequence suggested at least three vitellogenin (VTG) zona radiata protein (ZRP) were represented. Clones unique (62 up-, 13 down-regulated) arrayed as probes on nylon membranes investigate temporal expression oestrogen-responsive experimental animals. Arrays hybridised radiolabelled cDNAs prepared hepatic mRNA animals treated EE2 for various times upto 21 days transferred clean water a further 31 days. By day treatment 11 out 17 unidentified genes, 21/22 VTG, 13/14 ZRP, 2/2 aspartic proteinase (LAP) 8/10 other gene sequences induced by exposure. Of down-regulated sequences, only showed significant, decreased these encode cytochrome b two cryptic functions. Based pattern response up-regulated fell into classes. Pattern A reached maximum 16 exposure then declined prior removal B maximal between 22, declining after EE2. Independent investigation patterns selected using quantitative Real-Time PCR reproduced distinctive patterns. The results indicate previously unrecognised mechanism oestrogenic toxicity which there is selective down-regulation some egg proteins, potentially diminishing quality eggs this may contribute reproductive failure described elsewhere.
elsevier.com
sci-hub.se 参考文章(52)
D H Van Thiel, R H Kelly, P K Eagon, B Lombardi, K Okamura, J S Gavaler, K N Rao, Acute hemorrhagic pancreatic necrosis in mice. Induction in male mice treated with estradiol. American Journal of Pathology. ,vol. 109, pp. 8- 14 ,(1982)
Hiroko Shike, Xavier Lauth, Mark E Westerman, Vaughn E Ostland, James M Carlberg, Jon C Van Olst, Chisato Shimizu, Philippe Bulet, Jane C Burns, None, Bass hepcidin is a novel antimicrobial peptide induced by bacterial challenge FEBS Journal. ,vol. 269, pp. 2232- 2237 ,(2002) , 10.1046/J.1432-1033.2002.02881.X
Susan Jobling, Monique Nolan, Charles R. Tyler, Geoff Brighty, John P. Sumpter, Widespread Sexual Disruption in Wild Fish Environmental Science & Technology. ,vol. 32, pp. 2498- 2506 ,(1998) , 10.1021/ES9710870
G.J. Van Der Kraak, K.R. Munkittrick, M.E. McMaster, C.B. Portt, J.P. Chang, Exposure to bleached kraft pulp mill effluent disrupts the pituitary-gonadal axis of white sucker at multiple sites Toxicology and Applied Pharmacology. ,vol. 115, pp. 224- 233 ,(1992) , 10.1016/0041-008X(92)90327-O
Rubén Claudio Aguilar, Beverly Wendland, Ubiquitin: not just for proteasomes anymore Current Opinion in Cell Biology. ,vol. 15, pp. 184- 190 ,(2003) , 10.1016/S0955-0674(03)00010-3
Trevor P. Rodgers-Gray, Susan Jobling, Carole Kelly, Steven Morris, Geoff Brighty, Michael J. Waldock, John P. Sumpter, Charles R. Tyler, Exposure of Juvenile Roach (Rutilus Rutilus) to Treated Sewage Effluent Induces Dose-Dependent and Persistent Disruption in Gonadal Duct Development Environmental Science & Technology. ,vol. 35, pp. 462- 470 ,(2001) , 10.1021/ES001225C
H. K. Bhat, G. Calaf, T. K. Hei, T. Loya, J. V. Vadgama, Critical role of oxidative stress in estrogen-induced carcinogenesis Proceedings of the National Academy of Sciences of the United States of America. ,vol. 100, pp. 3913- 3918 ,(2003) , 10.1073/PNAS.0437929100
Christian Saligaut, Boris Linard, Evaristo L. Mañanos, Olivier Kah, Bernard Breton, Marina Govoroun, Release of pituitary gonadotrophins GtH I and GtH II in the rainbow trout (Oncorhynchus mykiss): modulation by estradiol and catecholamines. General and Comparative Endocrinology. ,vol. 109, pp. 302- 309 ,(1998) , 10.1006/GCEN.1997.7033
Rupesh P Amin, Hisham K Hamadeh, Pierre R Bushel, Lee Bennett, Cynthia A Afshari, Richard S Paules, Genomic interrogation of mechanism(s) underlying cellular responses to toxicants. Toxicology. ,vol. 181, pp. 555- 563 ,(2002) , 10.1016/S0300-483X(02)00481-X
Josephine A. Hagger, Andrew S. Fisher, Stephen J. Hill, Michael H. Depledge, Awadhesh N. Jha, Genotoxic, cytotoxic and ontogenetic effects of tri-n-butyltin on the marine worm, Platynereis dumerilii (Polychaeta: Nereidae). Aquatic Toxicology. ,vol. 57, pp. 243- 255 ,(2002) , 10.1016/S0166-445X(01)00200-4