Interactions of Escherichia coli RNA with bacteriophage MS2 coat protein: genomic SELEX

摘要: Genomic SELEX is a method for studying the network of nucleic acid–protein interactions within any organism. Here we report discovery several interesting and potentially biologically important using genomic SELEX. We have found that bacteriophage MS2 coat protein binds Escherichia coli mRNA fragments more tightly than it natural, well-studied, phage site. from rffG (involved in formation lipopolysaccharide bacterial outer membrane), ebgR (lactose utilization repressor), as well other genes. may yield experimentally induced artifacts, such molecules which fixed sequences participate binding. describe methods (annealing oligonucleotides complementary to or switching sequences) eliminate some, almost all, these artifacts. Such be useful tools both randomized sequence