A dot enzyme-linked immunosorbent assay for detection of antibodies against Entamoeba histolytica
作者： Sanjai Kumar , A.H. Band , J.C. Samantaray , N. Dang , G.P. Talwar
DOI: 10.1016/0022-1759(85)90065-1
关键词:
摘要: Abstract A visual micro-dot enzyme-linked immunosorbent assay (ELISA) based on detection of antibodies against soluble antigens axenically grown cultures Entamoeba histolytica is described. The antigen was spotted a nitrocellulose sheet, the unsaturated sites blocked with bovine serum albumin (BSA) and incubated 3-fold dilutions patient sera followed by incubation protein conjugated to peroxidase. Enzymic activity evidenced using substrate 4-chloro-1-naphthol. positive reaction produced blue spot. sensitivity better comparable indirect haemag-glutination (IHA) plate ELISA. entire could be completed within 3 h. Antigen-loaded pre-blocked strips stored up months at room temperature 37°C.
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