Effects of L2C leukemia on macrophage-mediated responses

摘要: Preliminary experiments have suggested that guinea pig L2C B-cell leukemia cells were able to evade macrophage-mediated lysis. To determine whether the resistant macrophage cytotoxic activity or factors associated with contributed a generalized inhibition of activity, pulmonary macrophages from strain 2 pigs tested for their ability lyse susceptible K562 cell line after activation by lipopolysaccharide (LPS) lymphokines. In addition, potential presence soluble inhibitors tumoricidal in serum-free culture supernatants and serum terminally ill was determining effects leukemic (LGPS) L2C-conditioned medium (CM) on normal macrophages. Macrophages demonstrated be depressed against stimulation either LPS lymphokines when compared The lymphokine-stimulated inhibited LGPS CM. Oxidative burst macrophages, as measured zymosan-stimulated production superoxide hydrogen peroxide, also under these conditions. data presented here suggests can suppress oxidative vitro this effect may contribute cytotoxicity.