X-ray crystal structure of Escherichia coli HspQ, a protein involved in the retardation of replication initiation.

作者: Yoshito Abe , Seijiro Shioi , Shunsuke Kita , Hikaru Nakata , Katsumi Maenaka

DOI: 10.1002/1873-3468.12892

关键词:

摘要: The heat shock protein HspQ (YccV) of Escherichia coli has been proposed to participate in the retardation replication initiation cells with dnaA508 allele. In this study, we have determined 2.5-A-resolution X-ray structure trimer HspQ, which is also first a member YccV superfamily. acidic character suggests an interaction surface basic proteins. From these results, discuss cellular function including its relationship DnaA508 protein. This article protected by copyright. All rights reserved.

参考文章(58)
Flemming G. Hansen, Sos Koefoed, Tove Atlung, Cloning and nucleotide sequence determination of twelve mutant dnaA genes of Escherichia coli. Molecular Genetics and Genomics. ,vol. 234, pp. 14- 21 ,(1992) , 10.1007/BF00272340
H. Blüthmann, L. Cicurel, G.W. Kuntz, G. Haedenkamp, K. Illmensee, Immunohistochemical localization of mouse testis‐specific phosphoglycerate kinase (PGK‐2) by monoclonal antibodies. The EMBO Journal. ,vol. 1, pp. 479- 484 ,(1982) , 10.1002/J.1460-2075.1982.TB01194.X
F Gotfried, J A Wechsler, Dominance of dnaA+ to dnaA in Escherichia coli. Journal of Bacteriology. ,vol. 130, pp. 963- 964 ,(1977) , 10.1128/JB.130.2.963-964.1977
A. Kornberg, Deog Su Hwang, E. Crooke, Aggregated dnaA protein is dissociated and activated for DNA replication by phospholipase or dnaK protein. Journal of Biological Chemistry. ,vol. 265, pp. 19244- 19248 ,(1990) , 10.1016/S0021-9258(17)30650-6
Anastassis Perrakis, Richard Morris, Victor S. Lamzin, Automated protein model building combined with iterative structure refinement. Nature Structural & Molecular Biology. ,vol. 6, pp. 458- 463 ,(1999) , 10.1038/8263
Zbyszek Otwinowski, Wladek Minor, Processing of X-ray diffraction data collected in oscillation mode Methods in Enzymology. ,vol. 276, pp. 307- 326 ,(1997) , 10.1016/S0076-6879(97)76066-X
Bjarke Bak Christensen, Tove Atlung, Flemming G. Hansen, DnaA boxes are important elements in setting the initiation mass of Escherichia coli. Journal of Bacteriology. ,vol. 181, pp. 2683- 2688 ,(1999) , 10.1128/JB.181.9.2683-2688.1999
J. Marszalek, J.M. Kaguni, DnaA protein directs the binding of DnaB protein in initiation of DNA replication in Escherichia coli. Journal of Biological Chemistry. ,vol. 269, pp. 4883- 4890 ,(1994) , 10.1016/S0021-9258(17)37627-5
Toh-ru Shimuta, Kiyotaka Nakano, Yoko Yamaguchi, Shogo Ozaki, Kazuyuki Fujimitsu, Chika Matsunaga, Kenji Noguchi, Akiko Emoto, Tsutomu Katayama, Novel heat shock protein HspQ stimulates the degradation of mutant DnaA protein in Escherichia coli Genes to Cells. ,vol. 9, pp. 1151- 1166 ,(2004) , 10.1111/J.1365-2443.2004.00800.X