Activated H-ras Oncogenes in Human Kidney Tumors
作者： Matthias H. Kraus , Yoshitaka Ebi , Hitoshi Onoue , Yukihiko Kitamura , Shiv K. Srivastava
DOI:
关键词:
摘要: Two H- ras oncogenes were detected by NIH/3T3 transfection assay out of 16 primary kidney tumors, 15 renal cell carcinomas (RCC), and one transitional carcinoma in patients. Analysis M r 21,000 protein suggested single point mutations within codon 12 61 each case. The restriction endonuclease analysis gene at confirmed this them, the remaining tumors did not have a mutation site. DNAs from noncancerous portions with mutated tumor, but leukocytes same patient, showed an abnormal resistance to endonucleases Msp I Hpa II, suggesting presence cells. No amplification genes was analyzed. In eight patients heterozygous for related Bam HI fragments, allele lost tumor portion kidney. Although cytogenetic studies previously nonrandom involvement c- raf -1 RCC, no abnormality size nor amount transcript RCCs. Our results thus indicated that genetic lesions affecting do occur human probably serve as multisteps carcinogenic process.
aacrjournals.org参考文章(14)
Andrew J. Cohen, Frederick P. Li, Solomon Berg, David J. Marchetto, Shien Tsai, Stephen C. Jacobs, Robert S. Brown, Hereditary Renal-Cell Carcinoma Associated with a Chromosomal Translocation New England Journal of Medicine. ,vol. 301, pp. 592- 595 ,(1979) , 10.1056/NEJM197909133011107
Johannes L. Bos, Deniz Toksoz, Christopher J. Marshall, Matty Verlaan-de Vries, Gerrit H. Veeneman, Alex J. van der Eb, Jacques H. van Boom, Johannes W. G. Janssen, Ada C. M. Steenvoorden, Amino-acid substitutions at codon 13 of the N- ras oncogene in human acute myeloid leukaemia Nature. ,vol. 315, pp. 726- 730 ,(1985) , 10.1038/315726A0
Larry Kedes, Joanne N. Engel, Peter W. Gunning, Isolation and characterization of human actin genes ,(1982)
E. Premkumar Reddy, Roberta K. Reynolds, Eugenio Santos, Mariano Barbacid, A point mutation is responsible for the acquisition of transforming properties by the T24 human bladder carcinoma oncogene Nature. ,vol. 300, pp. 149- 152 ,(1982) , 10.1038/300149A0
Eric R. Fearon, Andrew P. Feinberg, Stanley H. Hamilton, Bert Vogelstein, Loss of genes on the short arm of chromosome 11 in bladder cancer Nature. ,vol. 318, pp. 377- 380 ,(1985) , 10.1038/318377A0
S. K. Srivastava, Y. Yuasa, S. H. Reynolds, S. A. Aaronson, Effects of two major activating lesions on the structure and conformation of human ras oncogene products. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 82, pp. 38- 42 ,(1985) , 10.1073/PNAS.82.1.38
R. Weinberg, The action of oncogenes in the cytoplasm and nucleus Science. ,vol. 230, pp. 770- 776 ,(1985) , 10.1126/SCIENCE.2997917
H. Sakamoto, M. Mori, M. Taira, T. Yoshida, S. Matsukawa, K. Shimizu, M. Sekiguchi, M. Terada, T. Sugimura, Transforming gene from human stomach cancers and a noncancerous portion of stomach mucosa. Proceedings of the National Academy of Sciences. ,vol. 83, pp. 3997- 4001 ,(1986) , 10.1073/PNAS.83.11.3997
John M Chirgwin, Alan E Przybyla, Raymond J MacDonald, William J Rutter, None, Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. Biochemistry. ,vol. 18, pp. 5294- 5299 ,(1979) , 10.1021/BI00591A005
Kenneth Brown, Miguel Quintanilla, Martin Ramsden, Ian B. Kerr, Sheena Young, Allan Balmain, v-ras genes from harvey and BALB murine sarcoma viruses can act as initiators of two-stage mouse skin carcinogenesis Cell. ,vol. 46, pp. 447- 456 ,(1986) , 10.1016/0092-8674(86)90665-3