The Current State of the Golgi Proteomes

摘要: The Golgi apparatus plays a central role in the eukaryotic secretory pathway shuttling products between variety of destinations throughout cell. It is major site for post translational modification and processing proteins as well having significant synthesis complex carbohydrates. exists contiguous component endomembrane system which encompasses endoplasmic reticulum (ER), plasma membrane, vacuoles, endosomes lysozymes (Morre & Mollenhauer, 2009). tightly linked to numerous signaling processes through membrane vesicular trafficking endomembrane. This interconnection provides communication recycling networks apparatus, vacuoles lysosomes. Thus, represents structure within cell by regulating an array biosynthetic processes. was first described at end 19th century Camillo using light microscopy on nerve tissue samples (Golgi, 1898; Droscher, 1998). A half passed with development electron microscope more detailed picture emerged highlighting extreme complexity heterogeneity organelle (Dalton Felix, 1953). classic that distinct membranous stack disassociated cytosol (Fig.1). familiar image conceals underlying interconnected nature this With last decade routine mass spectrometry-based proteomics, applying these methods functionally characterize biological systems has been focus. integrated associated makes analysis one most complicated subcellular compartments address modern proteomics techniques. chapter will highlight recent advances our knowledge about have largely driven isolation procedures subsequent proteomic analysis.