ANTISENSE OLIGONUCLEOTIDES SUPPRESSING EXPRESSION OF CHOLINESTERASE GENES MODULATE HEMATOPOIESIS IN VIVO AND EX VIVO

摘要: Antisense oligonucleotides are short, synthetic DNA chains designed to match the sequence of their target RNA in opposite orientation [hence “antisense” (Stein & Cheng, 1993); Fig. 1]. They often protected against degradation by introducing sulfur atoms internucleotidic bonds form phosphorothioates (Eckstein, 1985). actively taken up cells, where they find mRNA and associate with them RNA:DNA double-strands. Phosphorothioated further induce, within these a specific ribonuclease - RNase H, selectively destroying double stranded chains. Therefore, antisense drugs can prevent from being translated into protein at least two ways physical interference translation machinery initiation destruction. Experimental currently tested HIV patients, destroy viral RNA, leukemias, targetted towards onco-genes, many other diseases bone marrow 1993). Bone cells particularly convenient targets for drugs, as reached vivo an hour injection time. Moreover, proliferate rapidly differentiate more mature forms, making susceptible rapid changes gene expression.