VEGF induces airway epithelial cell proliferation in human fetal lung in vitro.
作者： Karen R. S. Brown , Katherine M. England , Kelli L. Goss , Jeanne M. Snyder , Michael J. Acarregui
DOI: 10.1152/AJPLUNG.2001.281.4.L1001
关键词:
摘要: Vascular endothelial growth factor (VEGF) is a potent cell mitogen involved in normal and abnormal angiogenesis. VEGF mRNA protein are abundant distal epithelium of midtrimester human fetal lung. In the present study, we identified immunoreactivity for KDR, major VEGF-specific receptor, lung epithelial cells tissue, suggesting possible autocrine or paracrine regulatory role pulmonary differentiation. Addition exogenous to explants resulted increased volume density lumen tissues, both morphometric parameters tissue Cellular proliferation demonstrated by bromodeoxyuridine uptake was prominent airway VEGF-treated explants. also surfactant (SP) A mRNA, SP-C SP-A levels compared with controls. However, SP-B were unaffected treatment. [(3)H]choline incorporation into total phosphatidylcholine treatment, but disaturated not affected VEGF. Based on these observations, conclude that may be an important developing
sci-hub.se 参考文章(49)
Nick J. Gonchoroff, Jerry A. Katzmann, Rose M. Currie, Elizabeth L. Evans, David W. Houck, Bruce C. Kline, Philip R. Greipp, Michael R. Loken, S-phase detection with an antibody to bromodeoxyuridine. Role of DNase pretreatment Journal of Immunological Methods. ,vol. 93, pp. 97- 101 ,(1986) , 10.1016/0022-1759(86)90438-2
J A Whitsett, T E Weaver, M A Lieberman, J C Clark, C Daugherty, Differential effects of epidermal growth factor and transforming growth factor-beta on synthesis of Mr = 35,000 surfactant-associated protein in fetal lung. Journal of Biological Chemistry. ,vol. 262, pp. 7908- 7913 ,(1987) , 10.1016/S0021-9258(18)47654-5
J A Whitsett, T E Weaver, J C Clark, N Sawtell, S W Glasser, T R Korfhagen, W M Hull, Glucocorticoid enhances surfactant proteolipid Phe and pVal synthesis and RNA in fetal lung. Journal of Biological Chemistry. ,vol. 262, pp. 15618- 15623 ,(1987) , 10.1016/S0021-9258(18)47771-X
S W Glasser, T R Korfhagen, T E Weaver, J C Clark, T Pilot-Matias, J Meuth, J L Fox, J A Whitsett, cDNA, deduced polypeptide structure and chromosomal assignment of human pulmonary surfactant proteolipid, SPL(pVal). Journal of Biological Chemistry. ,vol. 263, pp. 9- 12 ,(1988) , 10.1016/S0021-9258(19)57347-1
B Barleon, S Sozzani, D Zhou, HA Weich, A Mantovani, D Marme, Migration of human monocytes in response to vascular endothelial growth factor (VEGF) is mediated via the VEGF receptor flt-1 Blood. ,vol. 87, pp. 3336- 3343 ,(1996) , 10.1182/BLOOD.V87.8.3336.BLOODJOURNAL8783336
Thomas N George, Jeanne M Snyder, Regulation of surfactant protein gene expression by retinoic acid metabolites. Pediatric Research. ,vol. 41, pp. 692- 701 ,(1997) , 10.1203/00006450-199705000-00015
Takashi Omura, Keiji Miyazawa, Arne Östman, Carl-Henrik Heldin, Identification of a 190-kDa vascular endothelial growth factor 165 cell surface binding protein on a human glioma cell line. Journal of Biological Chemistry. ,vol. 272, pp. 23317- 23322 ,(1997) , 10.1074/JBC.272.37.23317
Michael J. Acarregui, Scott T. Penisten, Kelli L. Goss, Kristie Ramirez, Jeanne M. Snyder, Vascular Endothelial Growth Factor Gene Expression in Human Fetal Lung In Vitro American Journal of Respiratory Cell and Molecular Biology. ,vol. 20, pp. 14- 23 ,(1999) , 10.1165/AJRCMB.20.1.3251
Marina Guerrin, Hafida Moukadiri, Pascale Chollet, Fraņoise Moro, Kamla Dutt, Fraņois Malecaze, Jean Plouët, VASCULOTROPIN/VASCULAR ENDOTHELIAL GROWTH FACTOR IS AN AUTOCRINE GROWTH FACTOR FOR HUMAN RETINAL PIGMENT EPITHELIAL CELLS CULTURED IN VITRO Journal of Cellular Physiology. ,vol. 164, pp. 385- 394 ,(1995) , 10.1002/JCP.1041640219
T. J. Breen, Jerrold H. Zar, Biostatistical Analysis (2nd ed.). Journal of the American Statistical Association. ,vol. 81, pp. 577- ,(1986) , 10.2307/2289268