Expression of vascular endothelial growth factor in synovial fibroblasts is induced by hypoxia and interleukin 1beta.

摘要: Objective. To study the mechanism by which hypoxia and inflammatory cytokines mediate angiogenesis in rheumatoid pannus through their effects on fibroblast-like type B synoviocyte, major cell of normal synovia. Methods. Fibroblasts were prepared from synovial tissue healthy diseased individuals cultured presence various stimuli. The expression vascular endothelial growth factor (VEGF) was assessed ELISA reverse transcription polymerase chain reaction. Results. Unlike fibroblasts, fibroblasts arthritis (RA) osteoarthritis constitutively secreted significant levels VEGF, is known to act directly cells. VEGF secretion further inducible both interleukin 1β (IL-1β) these increases additive. In contrast, tumor necrosis α unable induce expression. Conclusion. Under or IL-1 stimulation, conditions common inflamed synovium, synoviocytes secrete increased likely nearby endothelia, promoting angiogenesis. constitutive may reflect an altered phenotype involved pathology RA.