Immunological features and the ability of inhibitory effects on enzymatic activity of an epitope vaccine composed of cholera toxin B subunit and B cell epitope from Helicobacter pylori urease A subunit.
作者： Le Guo , Xiaokang Li , Feng Tang , Yunmian He , Yingying Xing
DOI: 10.1007/S00253-011-3726-0
关键词:
摘要: Epitope vaccine based on urease of Helicobacter pylori is a promising option for prophylactic and therapeutic vaccination against H. infection. In this study, we constructed an epitope with mucosal adjuvant cholera toxin B subunit (CTB) (UreA183-203) A named CTB-UA. The CTB-UA fusion protein was expressed in Escherichia coli, the purified used intraperitoneal immunization experiments BALB/c mice. experimental results indicated that anti-CTB-UA antibody could recognize both (UreA) (UreB). Besides, had good immunogenicity immunoreactivity induce specific neutralizing antibodies which showed effectively inhibitory effect enzymatic activity urease. molecule to be investigated as antigen candidate.
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