RNA-Seq-based analysis of differential gene expression associated with hepatitis C virus infection in a cell culture.

摘要: Hepatitis C virus (HCV) infections are one of the major causes chronic liver diseases. Unfortunately, mechanisms HCV infection-induced injury still not well recognized. Thus, identification and better characterization host cell response to infection may bring valuable insights into HCV-induced cytopathic effect host-virus interactions. To understand these processes we determined changes in gene expression that occur during Huh-7.5 cells. As a result, identified genes contribute cellular immune metabolic infection. Pathway enrichment analysis indicated induced increased involved mitogen-activated protein kinases signaling, adipocytokine cycle nitrogen metabolism. In addition, analyses molecular functions revealed up-regulated were implicated mainly negative regulation phosphorylation. The construction pathway-gene-process network enabled exploration much more complex landscape Consequently, several essential altered by identified: cycle, endoplasmic reticulum stress, reactive oxygen species, toll-like receptor signaling pattern recognition signaling. whose was decreased upon showed latter engaged metabolism lipids amino acids. Moreover, observed disturbance antiviral defense. Altogether, our results demonstrated elicits includes very wide range mechanisms.