Effective photodynamic therapy against microbial populations in human deep tissue abscess aspirates

摘要: Background and Objective The primary therapy for deep tissue abscesses is drainage accompanied by systemic antimicrobial treatment. However, the long antibiotic course required increases probability of acquired resistance, high incidence polymicrobial infections in complicates treatment choices. Photodynamic (PDT) effective against multiple classes organisms, including those displaying drug may serve as a useful adjunct to standard care reduction abscess microbial burden following drainage. Study Design/Materials Methods Aspirates were obtained from 32 patients who underwent image-guided percutaneous cavity. The majority specimens (24/32) abdominal, with remainder liver lung. Conventional microbiological techniques nucleotide sequence analysis rRNA gene fragments used characterize populations aspirates. We evaluated sensitivity microorganisms methylene blue-sensitized PDT vitro both within context an aspirate individual isolates. Results Most isolates bacterial, fungus Candida tropicalis also isolated two specimens. examined these blue-PDT. Complete elimination culturable was achieved three different aspirates, significant killing (P < 0.0001) observed all tested compared controls. Conclusions These results technical feasibility advancing optical fibers through catheters at time motivate further work on therapeutic option during Lasers Surg. Med. 45:509–516, 2013. © 2013 Wiley Periodicals, Inc.