Construction of defined polytopic integral transmembrane proteins. The role of signal and stop transfer sequence permutations.
作者： R E Rothman , D W Andrews , M C Calayag , V R Lingappa
DOI: 10.1016/S0021-9258(19)81540-5
关键词:
摘要: Signal and stop transfer sequences are discrete regions within a polypeptide chain able to initiate or terminate translocation of the protein across membrane endoplasmic reticulum. We have investigated role these topogenic in biogenesis polytopic transmembrane proteins. Plasmids encoding various patterns well-characterized signal fused set topogenically inert passenger domains were constructed. These molecules expressed by transcription-translation cell-free system microinjection transcripts into Xenopus oocytes. The observed orientation with respect was dependent on order coding region. results used test hypothesis that can achieve using combinations simple sequences. conclude some (but not all) confer proteins
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