Stabilized bioactive peptides and methods of identification, synthesis, and use

摘要: An intracellular selection system allows screening for peptide bioactivity and stability. Randomized recombinant peptides are screened in a tightly regulated expression system, preferably derived from the wild-type lac operon. Bioactive thus identified inherently protease- peptidase-resistant. Also provided bioactive stabilized by stabilizing group at N-terminus, C-terminus, or both. The can be small stable protein, such as Rop glutathione sulfotransferase, thioredoxin, maltose binding reductase, an α-helical moiety, one more proline residues.