Gene promoter hypermethylation is found in sentinel lymph nodes of breast cancer patients, in samples identified as positive by one-step nucleic acid amplification of cytokeratin 19 mRNA.

摘要: We analysed the promoter methylation status of five genes, involved in adhesion (EPB41L3, TSLC-1), apoptosis (RASSF1, RASSF2) or angiogenesis (TSP-1), intraoperative sentinel lymph node (SLN) biopsy samples from patients with breast cancer, that had been processed by one-step nucleic acid amplification (OSNA) technique. SLN resection is performed to estimate risk tumour cells clinically negative axilla, avoid unnecessary axillary dissection. OSNA currently one eligible molecular methods for detecting SLNs. It based on quantitative evaluation cytokeratin 19 mRNA which allows distinguishing between macrometastasis, micrometastasis and isolated cells, basis quantity present. There have no prior studies question whether not can be used further studies, including epigenetic abnormalities are some most important alterations cancer. Genomic DNA was extracted obtained 50 diagnosed primary The content SLNs evaluated OSNA, selected genes methylation-specific PCR. All were found hypermethylated a variable degree, RASSF1 hypermethylation significantly associated (p = 0.002). show suitable gene methylation. These provide new source material identification additional biomarkers.