Method of minimizing contamination in amplification reactions using a reaction tube with a penetrable membrane

摘要: A disposable reaction vessel for performing nucleic acid amplification assay. The has a penetrable cap that can be penetrated by an automated pipettor to aspirate portion of amplified product. contains the reagents necessary perform patient specimen is added unit dose in and closed. containing mixture undergoes amplification, typically placing it thermal cycler. After intact transferred analyzer where penetrates closure membrane aspirates sample further processing, without removal cap. This avoids generation potentially contaminating aerosols or droplets.