Ex-vivo application of solid microparticulate therapeutic agents

摘要: The present invention is concerned with a method of preparing and delivering small particles pharmaceutically active material to mammalian subject for treating diseases or disorders. A preferred embodiment entails: (i) the collection tissue cells from an animal donor, (ii) selective non-selective growth these in cell culture medium which added solid therapeutically compound, mostly free drug carrier (about 10% less, by weight), having average particle size less than about 100 microns, (iii) contacting compound causing be taken up into either intracellular compartment cultured cells, attachment as periphery such combination uptake surface, (iv) optionally, isolation and/or resuspension prepared steps i through iii, (v) administering subject. can administered intravenously, intramuscularly, subcutaneously, intradermally, intra-articularly, intrathecally, epidurally, intracerebrally, via buccal route, rectally, topically, transdermally, orally, intranasally, pulmonary intraperitoneally, combinations threof. After administration, loaded transport pharmaceutical composition particles.