A Multifaceted Study of Scedosporium boydii Cell Wall Changes during Germination and Identification of GPI-Anchored Proteins

摘要: Scedosporium boydii is a pathogenic filamentous fungus that causes wide range of human infections, notably respiratory infections in patients with cystic fibrosis. The development new therapeutic strategies targeting S. necessitates better understanding the physiology this and identification molecular targets. In work, we studied conidium-to-germ tube transition using variety techniques including scanning transmission electron microscopy, atomic force two-phase partitioning, microelectrophoresis cationized ferritin labeling, chemical spectroscopy, lectin nanoLC-MS/MS for cell wall GPI-anchored protein analysis. We demonstrated undergoes structural changes germination accompanied lower hydrophobicity, electrostatic charge binding capacity to ferritin. Changes during also included higher accessibility some polysaccharides lectins less CH3/CH3 interactions (hydrophobic adhesion forces mainly due glycoproteins). extracted identified 20 proteins from boydii, among which one was detected only conidial extract 12 mycelial extract. sequences belonged families involved virulence other fungi like Gelp/Gasp, Crhp, Bglp/Bgtp superoxide dismutase. These results highlighted remodeling substantial number or hyphal specific proteins, provides basis investigate role these molecules host-pathogen interaction fungal virulence.