High-throughput screening of cellular redox sensors using modern redox proteomics approaches.

摘要: Cancer cells are characterized by higher levels of intracellular reactive oxygen species (ROS) due to metabolic aberrations. ROS widely accepted as second messengers triggering pivotal signaling pathways involved in the process cell metabolism, cycle, apoptosis, and autophagy. However, underlying cellular mechanisms remain largely unknown. Recently, accumulating evidence has demonstrated that initiate redox through direct oxidative modification cysteines key redox-sensitive proteins (termed sensors). Uncovering functional changes regulation sensors is urgently required, role different distinct disease states still remains be identified. To assist this, proteomics been developed for high-throughput screening sensors, which will benefit development novel therapeutic strategies cancer treatment. Highlighted here recent advances approaches the...