作者: Gabriel G. Perrone , Ian W. Dawes , Anita Ayer , Julia Sanwald , Bethany A. Pillay
DOI: 10.1371/JOURNAL.PONE.0065240
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摘要: Responses to many growth and stress conditions are assumed act via changes the cellular redox status. However, direct measurement of pH-adjusted state during has never been carried out. Organellar (EGSH) was measured using fluorescent probes roGFP2 pHluorin in Saccharomyces cerevisiae. In particular, we investigated organellar response various better understand relationship between redox-, oxidative- environmental systems. EGSH values cytosol, mitochondrial matrix peroxisome were determined exponential stationary phase media. These (−340 −350 mV) more reducing than previously reported. Interestingly, sub-cellular remained unchanged when cells challenged with stresses reported affect homeostasis. Only hydrogen peroxide heat significantly altered state. Hydrogen glutathione disulfide/glutathione couple (GSSG, 2H+/2GSH) pH. Recovery from moderate most rapid followed by matrix, least able recover. Conversely, bulk shift observed resulted alterations pH not GSSG, 2H+/2GSH couple. This study presents first compartments conditions. Redox is distinctly regulated organelles data presented challenge notion that perturbation central