Distinct Redox Regulation in Sub-Cellular Compartments in Response to Various Stress Conditions in Saccharomyces cerevisiae

作者: Gabriel G. Perrone , Ian W. Dawes , Anita Ayer , Julia Sanwald , Bethany A. Pillay

DOI: 10.1371/JOURNAL.PONE.0065240

关键词:

摘要: Responses to many growth and stress conditions are assumed act via changes the cellular redox status. However, direct measurement of pH-adjusted state during has never been carried out. Organellar (EGSH) was measured using fluorescent probes roGFP2 pHluorin in Saccharomyces cerevisiae. In particular, we investigated organellar response various better understand relationship between redox-, oxidative- environmental systems. EGSH values cytosol, mitochondrial matrix peroxisome were determined exponential stationary phase media. These (−340 −350 mV) more reducing than previously reported. Interestingly, sub-cellular remained unchanged when cells challenged with stresses reported affect homeostasis. Only hydrogen peroxide heat significantly altered state. Hydrogen glutathione disulfide/glutathione couple (GSSG, 2H+/2GSH) pH. Recovery from moderate most rapid followed by matrix, least able recover. Conversely, bulk shift observed resulted alterations pH not GSSG, 2H+/2GSH couple. This study presents first compartments conditions. Redox is distinctly regulated organelles data presented challenge notion that perturbation central

参考文章(57)
Evgeniĭ Evgenʹevich Selʹkov, J. G. Reich, Energy Metabolism of the Cell: A Theoretical Treatise ,(1982)
Tova Zehavi-Willner, Edward M. Kosower, Tim Hunt, Nechama S. Kosower, Glutathione. V. The effects of the thiol-oxidizing agent diamide on initiation and translation in rabbit reticulocytes. Biochimica et Biophysica Acta. ,vol. 228, pp. 245- 251 ,(1971) , 10.1016/0005-2787(71)90564-8
P. K. Sorger, H. R. Pelham, Purification and characterization of a heat-shock element binding protein from yeast. The EMBO Journal. ,vol. 6, pp. 3035- 3041 ,(1987) , 10.1002/J.1460-2075.1987.TB02609.X
T DRAKULIC, M TEMPLE, R GUIDO, S JAROLIM, M BREITENBACH, P ATTFIELD, I DAWES, Involvement of oxidative stress response genes in redox homeostasis, the level of reactive oxygen species, and ageing in Saccharomyces cerevisiae. Fems Yeast Research. ,vol. 5, pp. 1215- 1228 ,(2005) , 10.1016/J.FEMSYR.2005.06.001
Gerald Cohen, Friederike Fessl, Aleksandra Traczyk, Joanna Rytka, Helmut Ruis, Isolation of the catalase A gene of Saccharomyces cerevisiae by complementation of the cta1 mutation. Molecular Genetics and Genomics. ,vol. 200, pp. 74- 79 ,(1985) , 10.1007/BF00383315
Anita Ayer, Shi-Xiong Tan, Chris M. Grant, Andreas J. Meyer, Ian W. Dawes, Gabriel G. Perrone, The critical role of glutathione in maintenance of the mitochondrial genome Free Radical Biology and Medicine. ,vol. 49, pp. 1956- 1968 ,(2010) , 10.1016/J.FREERADBIOMED.2010.09.023
James R. Matthews, Naomi Wakasugi, Jean-Louis virelizier, Junji Yodoi, Ronald T. Hay, Thioredoxin regulates the DNA binding activity of NF-kappa B by reduction of a disulphide bond involving cysteine 62. Nucleic Acids Research. ,vol. 20, pp. 3821- 3830 ,(1992) , 10.1093/NAR/20.15.3821
William C. Burhans, Nicholas H. Heintz, The cell cycle is a redox cycle: Linking phase-specific targets to cell fate Free Radical Biology and Medicine. ,vol. 47, pp. 1282- 1293 ,(2009) , 10.1016/J.FREERADBIOMED.2009.05.026