UPLC-Orbitrap HRMS metabolic profiling of Cymbopogon citratus cultivated in Egypt; neuroprotective effect against AlCl3-induced neurotoxicity in rats

摘要: Abstract Ethnopharmacological relevance Cymbopogon citratus (lemongrass) is commonly used in teas, soups and treat inflammatory-based ailments, vascular nervous disorders. Aim of the study The aimed to evaluate neuroprotective effect leaves through scientific protocol. aqueous (AE) ethanolic (EE) extracts was evaluated against AlCl3-induced Alzheimer's disease (AD) rats. Metabolic profiling plant, isolation bioactive compounds standardization active fraction were investigated. Materials methods AE prepared as per traditional method (infusion), EE by repeated maceration 90% ethanol, (BAEE) obtained from thereof column chromatography. performed UPLC-Orbitrap HRMS HPLC for standardization. rats assess extracts. Neuroprotective mechanism(s) clarified histopathological examination brain tissues, estimation AD biochemical markers, oxidative stress neuroinflammation homogenates. In addition, antioxidant (using DPPH assay) anticholinesterase modified Ellman's method) activities Results AlCl3-treated (17 mg/kg/day) showed alteration tissues together with elevated levels Aβ, tau proteins, MDA, NF-kB IL-6. However, treatment prevented pathological changes maintained inflammatory markers. BAEE significantly inhibited acetylcholinesterase enzyme (2.11 ± 0.11 mg/ml) exhibited a strong activity (24.99 ± 0.00 μg/ml). UPLC-MS peaks twenty-eight compounds, twenty-one them identified. Three flavonoids; isoorientin, isoschaftoside luteolin-7-O-neohesperidoside isolated major constituents. powdered found contain remarkable amounts caffeic acid (3.49 mg/g dry wt.) isoorientin (7.37 mg/g determined HPLC. Conclusion extract attenuates neurotoxicity inhibition This could possibly attributed, part its high content phenolic acids flavonoids. Accordingly, we recommend protection AD.