Methods for Separating Native Enzymes

作者: Gunter M. Rothe

DOI: 10.1007/978-3-642-79069-0_3

关键词:

摘要: In the course of electrophoresis stability an enzyme depends on such conditions as (a) pH-value, (b) ion strength and species, (c) effector molecules, (d) temperature (e) properties separation matrix. These parameters were empirically optimized for starch gel [1–3] cellulose acetate [4, 5] when analyzing predominantly animal human specimen. A major advantage these types media is that practically every buffer system can be used to separate enzymes whereas in disc-gel [6–8] number applicable systems limited. When using isoelectric focusing native no choice at all possible [9–10]. On other hand, more time consuming than electrophoresis. Cellulose membranes are commercially available easy handle, visualization together do not take longer 1–1.5 h. clinical diagnosis therefore, both methods favoured. Disc-gel electrophoresis, gradient if capacities insufficient or point molecular mass estimated. The data compiled Tables 3.1 3.2 decide between a particular case.

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