Cloned neuropeptide Y (NPY) Y1 and pancreatic polypeptide Y4 receptors expressed in Chinese hamster ovary cells show considerable agonist-driven internalization, in contrast to the NPY Y2 receptor.

摘要: Guinea-pig neuropeptide Y1 and rat pancreatic polypeptide Y4 receptors expressed in Chinese hamster ovary cells were internalized rapidly upon attachment of selective peptide agonists. The Y2, but not the Y4, receptor also nonselective Y agonist, human/rat Y. internalization guinea-pig Y2 was small at 37 degrees C, essentially absent or below 15 possibly connection to large molecular size receptor-ligand complexes (up 400 kDa for fraction). rate intake strongly temperature dependent, with no 6 C any receptor. Internalized largely associated light, endosome-like particulates. Sucrose dose-dependently decreased all receptors, while affecting ligand cell membrane sites much less. Internalization could be blocked, that significantly inhibited, by phenylarsine oxide, which unmasked spare cell-surface especially abundant subtype. restoration after agonist pretreatment cycloheximide monensin. Thus, have larger subcellular dynamics than This differential hold organismic systems, is comparable known differences angiotensin, bradykinin, somatostatin opioid subtypes.