The 6-kilodalton membrane protein of Semliki Forest virus is involved in the budding process.

摘要: Alphavirus genomes encode a small hydrophobic protein of 6 kDa (the 6K protein) that is expressed as part large polyprotein containing the sequences two virus transmembranal glycoproteins which form spikes infectious particle. Although made in amounts equivalent to those glycoproteins, very little found secreted virions. The role this replication and structure has been studied by use variety mutationally altered forms 6K, yield phenotypically distinct viruses. A complete deletion gene encoding (delta 6K) Semliki Forest Virus (SFV) constructed from an SFV cDNA transcribed RNA-produced progeny closely resembled normal (P. Liljestrom, S. Lusa, D. Huylebroeck, H. Garoff, J. Virol. 65:4107-4113, 1991). Further studies mutant have now performed, they show growth delta strong dependency on its host cell, varying 2 50% rate formation wild-type SFV. Mammalian cells are much more defective than insect avian mutant. This not transport or production nucleocapsids, accumulate at plasma cell membrane infected BHK cells. major defect, thus, final assembly budding new virus. In with strain, relatively fraction total formed can be recovered osmotic lysis exhaustively washed Infectious totally lacking identical early stages replication, i.e., binding uptake. particles themselves thermolabile SFV, suggesting may slower leads configuration trimeric spikes. These data support other implicate important but nonessential component alphavirus particle, perhaps affecting packing their interactions lipid.