Genome Editing in Escherichia coli with Cas9 and synthetic CRISPRs

摘要: Recently, the Cas9-CRISPR system has proven to be a useful tool for genome editing in eukaryotes, which repair double stranded breaks made by Cas9 with non-homologous end joining or homologous recombination. Escherichia coli lacks and very low recombination rate, effectively rendering targeted activity lethal. We have developed heat curable, serializable, plasmid based selectionless arbitrary E. strains that uses synthetic CRISPRs targeting -red effect repairs of breaks. demonstrated insertions, substitutions, multi-target deletions our system, we tested several strains.