A Novel Molecular Test to Diagnose Canine Visceral Leishmaniasis at the Point of Care.

摘要: Dogs are the principal reservoir hosts of zoonotic visceral leishmaniasis (VL) but current serological methods not sensitive enough to detect all subclinically infected animals, which is crucial VL control programs. Polymerase chain reaction (PCR) have greater sensitivity require expensive equipment and trained personnel, impairing its implementation in endemic areas. We developed a diagnostic test that uses isothermal recombinase polymerase amplification (RPA) Leishmania infantum. This method was coupled with lateral flow (LF) reading naked eye be adapted as point-of-care test. The L. infantum RPA-LF had an analytical similar real time-PCR, detecting DNA 0.1 parasites spiked dog blood, equivalent 40 parasites/mL. There no cross or human braziliensis, amazonensis, Trypanosoma cruzi. also amplified donovani strains (N = 7). In group clinically normal dogs 30), detected more subclinical infections than rK39 strip test, standard (50% versus 13.3% positivity, respectively; P 0.005). Also, noninvasive mucosal samples comparable blood samples. novel molecular may positive impact