Development of a new methodology for the detection of Colletotrichum truncatum and Fusarium sp. in bell pepper seed

摘要: This study was carried out towards the development of rapid PCR-based assays for detection and identification Colletotrichum truncatum, C. gloeosporioides sensu lato Fusarium sp. infecting green red bell pepper seeds in Trinidad using DNA templates produced by automated grinding robotic paramagnetic particle capture. extracted from freshly collected symptomatic Promega’s Maxwell® 16 system. The PCR assay 220 harvested field-infected fruit compared different optimization parameters including that were asymptomatic infection, relative success two polymerase enzymes, genus-specific species-specific primers pre-existing literature addition to newly designed this duplex simultaneous belonging fungal species. findings indicated dual infections truncatum Compared other screened, our primers, ITS219-F/ITS419-R with GoTaq® Green Master Hot Start Taq Polymerase, detected highest number infected as well fruit. only seeds. Duplex Fa/Ra successfully simultaneously identified None which is currently considered be a minor pathogen Trinidad. methods are key tools plant pathogens especially where an important inoculum source. molecular approach optimized study, faster, more sensitive presence single or can assessed one assay.