Establishment and mutation analysis of a novel malignant peritoneal mesothelioma cell line, TU-MM-1, using whole genome sequencing
作者： Nao Oumi , Hiroaki Itamochi , Hiroaki Komatsu , Tetsuro Oishi , Muneaki Shimada
DOI: 10.1007/S13577-015-0120-8
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摘要: A new cell line of human malignant peritoneal mesothelioma (MPM), TU-MM-1, was established and characterized. The cells showed polygonal morphology, grew in monolayers without contact inhibition were arranged like a jigsaw puzzle. chromosome numbers ranged from 41 to 44. low rate proliferation observed the doubling time 67.9 h. Genomic DNA sequencing revealed that TU-MM-1 harbored missense mutations APC, LATS2, BRCA1/2, TP53, mutation splice donor site BAP1 loss CDKN2A gene. We absence p16(INK4a) proteins, underexpression LATS2 protein, overexpression p53 protein western blot analysis. Heterotransplantation nude mice produced tumors had characteristics original tumor. This may be useful for studying biological properties contribute novel treatment strategies.
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