Hypericin-induced photosensitization of HeLa cells leads to apoptosis or necrosis Involvement of cytochrome c and procaspase-3 activation in the mechanism of apoptosis
作者： Annelies Vantieghem , Zerihun Assefa , Peter Vandenabeele , Wim Declercq , Stephane Courtois
DOI: 10.1016/S0014-5793(98)01416-1
关键词:
摘要: Here we report that photoactivated hypericin can induce either apoptosis or necrosis in HeLa cells. Under apoptotic conditions the cleavage of poly(ADP-ribose) polymerase (PARP) into 85-kDa product is blocked by caspase inhibitors benzyloxycarbonyl-Val-Ala-Asp-fluoromethylketone (z-VAD-fmk) and benzyloxycarbonyl-Asp-Glu-Val-Asp-fluoromethylketone (z-DEVD-fmk). Both protect cells from but cannot prevent hypericin-induced necrosis. Conversely, overexpressing viral cytokine response modifier A (CrmA), which inhibits caspase-1 -8, still undergo Evidence provided for release mitochondrial cytochrome c cytosol procaspase-3 activation cell killing.
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