Molecular cloning, characterization of CAT, and eco-toxicological effects of dietary zinc oxide on antioxidant enzymes in Eisenia fetida

摘要: The full-length cDNA of catalase (EfCAT) from Eisenia fetida was cloned (GenBank accession no. JN617999). Sequence characterization revealed that EfCAT protein sequence contained proximal heme-ligand signature (351RLFSYSDTH359), two glycosylation sites (N145 and N436), the active site (61FDRERIPERVVHAKGAGA78), 12 amino acids (N145, H191, F195, S198, R200, N210, Y212, K234, I299, W300, Q302, Y355), which were identified as putative residues involved in NADPH binding. These conserved motifs sequences essential for structure function EfCAT. present study also investigated effect veterinary food additive zinc oxide on antioxidant processes E. fetida, at different concentrations exposure durations. A significant increase (by 106.0 % compared to controls) CAT activity 500 mg/kg registered day 15. superoxide dismutase (SOD) increased maximum value 44.0 %) measured There a glutathione peroxidase (GPx) all after 5 days. results showed dietary Zn (500 mg/kg) causes oxidative damage earthworms. At early stages earthworms exposed ZnO, GPx is main enzyme impair status; while later enzymes SOD indicators stress. enzymatic variations may be an adaptive response survive contaminated soils.