Label-free differentiation of human pituitary adenomas by FT-IR spectroscopic imaging

摘要: Fourier transform infrared (FT-IR) spectroscopic imaging has been used to characterize different types of pituitary gland tumors and normal tissue. Freshly resected tumor tissue from surgery was prepared as thin cryosections examined by FT-IR imaging. Tissue were discriminated via k-means cluster analysis a supervised classification algorithm based on linear discriminant analysis. Spectral allowed us discriminate between non-tumor cells, well cells that produce human growth hormone (hGH+) do not (hGH−).The spectral compared contrasted with histological PAS orange G stained image. It further shown hGH+ show stronger amide bands than hGH. This study demonstrates can only potentially serve fast objective approach for discriminating tissue, but it also detect hGH-producing cells.