EFFECTS OF OUABAIN ON ACID‐SOLUBLE PHOSPHATES AND ELECTROLYTES OF ISOLATED CEREBRAL TISSUES IN PRESENCE OR ABSENCE OF CALCIUM

摘要: —(1) Cerebral slices were incubated in Ca2+-free media or which contained 2.8 mm-Ca2+. Omission of Ca2+ brought about a drop creatine phosphate content 28 per cent, as well 3–10 cent non-inulin K+ content. There was little change 10-min Na+. (2) Ouabain concentrations to M increased the loss from slice and caused rise Na+ The changes most marked media. Creatine levels depressed by ouabain both presence absence Ca2+. In Ca2+, lowering phosphocreatine did not occur until significant shifts had taken place. contrast, Ca2+-containing lost at same rate. (3) When labelled added simultaneously, there difference rate incorporation label into differed concentration. However, azP-labelled decreased 30–40 lacked when 15 min prior phosphate. This observed Ca2+. (4) affect oxidative phosphorylation respiratory control directly bovine brain mitochondrial preparations. (5) results suggest that previously depression respiration Ca2+-deficient is good indicator proportion cell's metabolism used for active cation transport. Under these conditions, inhibition transport accompanied high-energy may represent secondary effect ouabain, cytoplasmic alterations on energy-producing processes.