Quantitation of microorganisms associated with the particulate phase of ruminal ingesta.

摘要: Microbial organic matter associated with rumen particulate and fluid phases was quantified using 15N as the microbial marker in two fistulated cows fed a 65% alfalfa haylage diet. During collection periods, feed removed 1 h after initiation of feeding, were dosed (15NH4)2SO4. Whole contents sampled before feeding at various times up to 12 feeding. Fluid microorganisms those that passed through eight layers cheesecloth. Particle-associated obtained by chilling squeezed particles prior seven successive extractions saline solution. The amount from ranged 32.1 59.9% measured 15N. Organic (mg/mL strained equivalent) particle-associated respectively: 10.7 47.5; 12.5 35.5; 12.2 30.1; 26.1; 10.9 26.7; 8.9 20.6, 2, 3, 4, 7, 9 11 These ratios indicated 70-80% whole phase greatest soon Analysis extracted 50-65% particle nitrogen 17-27% dry origin. results provide evidence make major proportion total ruminal ingesta large N can be