Cryptic species and phylogeographical structure in the tree Cedrela odorata L. throughout the Neotropics

摘要: Aim: The origins of much Neotropical biodiversity remain a topic debate, with both palaeogeographical and more recent climatic drivers playing role in diversification among within species. Here we use combination molecular data to assess genetic variation species the tree genus Cedrela, focus on Cedrela odorata, test hypotheses diversification, place known ecotypic context detect intraspecific phylogeographical structure. Location: Central South America, Cuba, Cayman Islands, Trinidad Guadeloupe. Methods: Samples were collected from field, existing collections herbaria across geographical range, including total 528 individuals 72 sites. A phylogenetic framework was constructed using internal transcribed spacer (ITS) sequence (intergenic spacers plus flanking 18S 26S regions), structure analysed chloroplast DNA sequences (trnC–ycf6, trnH–psbA) nuclear microsatellite (single repeat, SSR) loci. Phylogenetic reconstruction undertaken Bayesian parsimony-based approaches; divergence times estimated for major nodes. Geographical SSR SAMOVA, while that assessed clustering principal coordinates analysis. Results: ITS supported distinctiveness four morphologically cryptic C. odorata. Chloroplast showed structuring species, suggesting influence drivers. Intraspecific also present data, contemporary gene flow limitation sea mountain barriers. Main conclusions: support America subsequent recolonization into prior formation Isthmus Panama. At least taxa evident odorata within-species Andes present, latter suggestive Pleistocene influence. Previously recognized ecotypes should be elevated level. new reclassification will monitoring exploitation genus.