Alpha-toxin is produced by skin colonizing Staphylococcus aureus and induces a T helper type 1 response in atopic dermatitis.

摘要: BACKGROUND Staphylococcus aureus is a well known trigger factor of atopic dermatitis (AD). Besides the superantigens, further exotoxins are produced by S. and may have an influence on eczema. OBJECTIVE To explore impact staphylococcal alpha-toxin human T cells, as those represent majority skin infiltrating cells in AD. METHODS Adult patients with AD were screened for cutaneous colonization producing aureus. As induce necrosis, CD4(+) incubated sublytic concentrations. Proliferation up-regulation IFN-gamma mRNA protein level assessed. The induction t-bet translocation was detected Electrophoretic Mobility Shift Assay. RESULTS Thirty-four percent colonized lesional these immunohistochemistry. Sublytic concentrations induced marked proliferation isolated cells. Microarray analysis indicated that particularly high amounts transcripts. Up-regulation confirmed both level. Stimulation resulted DNA binding t-bet, key transcription involved into primary helper type 1 (Th1) commitment. CONCLUSION from We show here first time activate absence antigen-presenting Our results indicate relevant Th1 like cytokine response. In AD, this facilitates development cell dominated chronic