A Key Role for Inhibins in Dendritic Cell Maturation and Function.

摘要: Inhibins are members of the TGFβ superfamily, which regulate many cellular processes including differentiation, proliferation, survival and apoptosis. Although initially described as hormones regulating hypothalamus-pituitary-gonadal axis, based on their ability to antagonize Activins, our group has recently reported that they play a role in thymocyte differentiation survival, well thymic stromal cell maturation nTreg generation. Here, we used Inhibin knock out mice (Inhα-/-) investigate peripheral dendritic function. We first demonstrated LPS treated Inhα+/+ bone marrow derived cells (BMDC) were capable produce significant levels A. Interestingly, Inhα-/- BMDC showed reduced MHCII CD86 upregulation increased PD-L1 expression response compared Inhα+/+, correlated with induce proliferation allogeneic T cells. The "semi-mature" phenotype displayed by mBMDC IL-10 slightly decreased IL-6 production after stimulation. In addition, impaired migration towards CCL19 CCL21, assessed vitro chemotaxis vivo competitive homing experiments, despite normal CCR7 expression. Furthermore, LPS-induced DC was also diminished mice, specially within LC (CD207+ CD11b+ CD103-) subpopulation. Finally, analysis delayed type hypersensitivity responses ear swelling result infiltration skin, correlating CD207+ DCs draining lymph nodes. summary, data demonstrate for time key function, balance between immunity tolerance.