Reproducibility of Proteomic Profiles Over 3 Years in Postmenopausal Women Not Taking Postmenopausal Hormones

摘要: Most proteomics studies examine one blood specimen per participant; however, it is unknown how well measures at time point reflect an individual9s long-term proteome pattern. Therefore, we examined the stability of over 3 years in postmenopausal women not taking hormones for least months using surface-enhanced laser desorption and ionization time-of-flight mass spectrometry. Using Nurses9 Health Study cohort, randomly selected 60 from a subset providing 2 to samples years. Four different protein chip surfaces/plasma fractions were examined: unfractionated plasma on CM10 H50 chip, pH ≥ 9, fraction organic all with low- high-energy transfer protocol. Participant quality control aligned reference sample then peak intensity was assessed peaks identified sample. The average coefficient variation (CV) within conditions ranged 16% (H50, organic, low protocol) 63% (CM10, high protocol). Generally, CV mean inversely correlated (median −0.48). intraclass correlation (ICC) 0.37 unfractionated, 0.68 For signal-to-noise cutoff 2.0, observed 334 peaks, which 241 (72%) had ICC ≥0.40. Although large range CVs ICCs, sufficient numbers reasonable ICCs suggest that reproducibility among hormones. (Cancer Epidemiol Biomarkers Prev 2008;17(6):1480–5)