Visualization of proteins with a silver “stain”: A critical analysis

摘要: Optimal silver staining of proteins in polyacrylamide gels is a fine balance between deposition on the and level background staining. Reproduciable requires empirical standardization many steps procedure. Silver present form does not stoichiometrically stain proteins, unlike Coomassie Blue. Staining intensity bear linear relationship to protein mass. This demonstrated by densitometric evaluation both Coomassie-stained marker fractioned sodium dodecly1 sulfate gel electrophoresis (SDS-PAGE). Although more sensitive than Blue, increment sensitivity different for individual dependent concentration. Nevertheless it an excellent method one- two-dimensional (2-D) if only minimal amounts are available qualitative or semi-quantitative required.