A simple liquid-chromatographic method for measuring vitamin B6 compounds in plasma.

摘要: This relatively simple high-performance liquid chromatographic (HPLC) method for measuring all seven known forms of vitamin B6 in plasma from individuals supplemented with pyridoxine hydrochloride shows good analytical recovery (85-98%) and precision. Within-run between-run CVs plasmas standards were 4% 7%, respectively. The major found unsupplemented normal subjects pyridoxal phosphate 4-pyridoxic acid, just detectable. HPLC procedure correlated well (r = 0.94) a modification an enzymatic involving apotryptophanase (Anal Biochem 1972;45:567-76) phosphate, also routine determining acid urine (Clin Chem 1964;10:479-89). Elimination the both hyperoxaluric was shown to be very rapid, half-lives (t1/2) 45 40 min, Finally, we present evidence existence two other discuss possibility new metabolic pathway metabolism.