Identification of distinct protein expression patterns in bilateral matched pair breast ductal fluid specimens from women with unilateral invasive breast carcinoma: High-throughput biomarker discovery

摘要: BACKGROUND Analysis of the biochemical and cellular contents breast ductal fluid has recently gained attention as a potential noninvasive method for studying local microenvironment associated with development progression carcinoma. METHODS Patients unilateral primary invasive carcinoma were eligible current prospective pilot study. Nipple aspiration (NAF) was obtained from cancer normal contralateral subjected to two-dimensional electrophoresis. Computer-assisted image analysis used analyze NAF protein expression profiles. RESULTS The number separate spots detected in samples ranged 1280 1649. Substantial qualitative differences identified between patterns compared without cancer. Protein not same patient varied 30 202 different proteins. In addition, 14 73 Conversely, an individual carcinoma, only three left but right breast, two breast. CONCLUSIONS The is unique organ that its can be readily accessed evaluated by nipple. Breast contains large As breasts are paired organs, comparisons patient's may reveal significant carcinoma. Recent advances analysis, automated mass spectrometry, bioinformatics have provided tools necessary use fluids patients high-throughput biomarker discovery. Cancer 2002;95:2276–82. © 2002 American Society. DOI 10.1002/cncr.10974