Hydrogen peroxide acts as relaxing factor in human vascular smooth muscle cells independent of map-kinase and nitric oxide

摘要: We previously showed that hydrogen peroxide (H2O2) induced resistance artery relaxation independent of endothelium. Thus, in this study we investigated the mechanism by H2O2 on human renal vascular smooth muscle cell (HVSMC). HVSMC were stimulated with and/or angiotensin II (Ang II), proline-rich-tyrosine-kinase-2 (PYK2), ERK1/2 MAP-Kinase, and myosin light chain 20 phosphorylation (Lc20) assessed using Western blot analysis presence potassium channel blockers, nitric oxide synthesis (NOS) inhibitors. increased PYK2 phosphorylation, at same time decreased Lc20 phosphorylation. AngII PYK2, Lc20, whereas, pretreatment AngII. MEK inhibition, but had no effect inhibition H2O2. The Ca2(+)-dependent K+ channels (BKCa) NOS did not block decrease response to On other hand, 60 mM KCl, rather than This shows evidence acts as a relaxing factor an activator Human VSMC. is BKCa, MAP-Kinase pathways. changes contracting when are compromised.