Inhibition of angiogenesis by anthracyclines and titanocene dichloride.

摘要: The anthracycline antibiotics, daunorubicin, doxorubicin, and epirubicin, which are widely used for treatment of malignancies, have been evaluated their effect on angiogenesis in relation to the inhibition collagenase type IV reported previously. In chick chorioallantoic membrane (CAM) system angiogenesis, anthracyclines inhibited vascular density at doses 5-20 micrograms/disc as well collagenous protein biosynthesis, is a reliable index angiogenesis. Similarly, all three tube formation vitro using human umbilical vein endothelial cells (HUVECs) plated Matrigel. was dose-dependent caused 50% concentrations 2.5-15 micrograms/mL. At prevented there were no cytotoxic effects, evidenced by methylene blue uptake, growth these not inhibited. experimental antitumor agent titanocene dichloride from Walker 256 carcinosarcoma with IC50 approximately 0.2 mM. Titanocene also CAM HUVECs Matrigel that without or cytotoxicity culture. antiangiogenic aforementioned agents therapeutically attainable may explain, least part, properties because an essential process tumor metastasis. is, however, unrelated metalloproteinase higher required than