Integrating Microstructured Electrospun Scaffolds in an Open Microfluidic System for in Vitro Studies of Human Patient-Derived Primary Cells.

摘要: Recent studies have suggested that microenvironmental stimuli play a significant role in regulating cellular proliferation and migration, as well modulating self-renewal differentiation processes of mammary cells with stem cell (SCs) properties. advances micro/nanotechnology biomaterial synthesis/engineering currently enable the fabrication innovative tissue culture platforms suitable for maintenance SCs vitro. Here, we report design an open microfluidic device (OMD) integrating removable poly(e-caprolactone) (PCL) based electrospun scaffolds, demonstrate OMD allows investigation behavior human during vitro real time. Electrospun scaffolds modified surface topography chemistry can influence attachment, proliferation, epigenetic mechanisms maintain luminal identity function specific morphological or biochemical cues imparted by tailor-made fiber post-treatments. Meanwhile, architecture control seeding conditions to collect more accurate informative assays. In perspective, integrated systems could be mimic physiological local microenvironment then analyze response from screening drugs effective diagnostics, long-term prognostics, disease intervention personalized medicine.