Overexpression of miR-595 and miR-1246 in the sera of patients with active forms of inflammatory bowel disease.

摘要: Background MicroRNAs (miRNAs) are dysregulated in the inflammatory bowel diseases, Crohn's disease (CD) and ulcerative colitis (UC), which arise due to dysfunctional host-microbe interactions impairment of barrier function intestine. Here, we sought determine whether circulating miRNAs biomarkers active colonic CD UC can provide insights into pathogenesis. Comparison was made with serum patients rheumatoid arthritis (RA). Methods Total RNA from CD, UC, RA, normal healthy adults screened for disease-associated by microarray analysis, subsequent validation quantitative reverse-transcription polymerase chain reaction. MiRNA targets were identified luciferase reporter assays. Results MiR-595 miR-1246 significantly upregulated sera RA patients, compared subjects; inactive disease. Luciferase assays indicated that miR-595 inhibits expression neural cell adhesion molecule-1 fibroblast growth factor receptor 2. Conclusions Serum RA. These findings gain significance reports impairs epithelial tight junctions, whereas indirectly activates proinflammatory nuclear activated T cells. molecule molecule-1, 2, plays a key role differentiation, protection, repair epithelium, maintenance junctions. warrant testing as potential therapeutic intervention treatment