Standardize plasma sample collection method for biomarker searching study in oncology

摘要: 22163 Background: Sample quality is critical for biomarker searching in oncology. Platelet degradation and contamination plasma has a remarkable impact on many blood markers. factor 4 (PF4) can be used as surrogate of this condition monitor the sample quality. This multicenter study aimed to validate handling process platelet degradation/contamination establish an optimal method poor plasma. Methods: Six centers participated. Blood were drawn from 12–13 healthy volunteers each center. samples prepared serum or by 9 methods (table). These sent University Michigan (American samples) Peking Union Medical College Cancer Hospital (Chinese measurement PF4 with ELISA. Paired-T test was statistic analysis. Results: A total 74 subjects enrolled. The generated lowest level ones 3000×g 30 min, set on...