Proteolytic Enzymes of Neurospora crassa
作者： Dietrich SIEPEN , Peter-Hao YU , Maria-Regina KULA
DOI: 10.1111/J.1432-1033.1975.TB02230.X
关键词:
摘要: Five intracellular proteolytic enzymes from Neurospora crassa were isolated and partially characterized: an acidic alkaline endopeptidase, one carboxypeptidase two aminopeptidases. All these proteinases purified the same crude extract to homogenity by heat treatment, precipitation with ammonium sulfate, chromatography on DEAE-cellulose, CM-cellulose, DEAE-Sephadex, hydroxyapatite gel filtration. The acid proteinase hydrolysed acid-denatured haemoglobin at pH 3.0. are serine that require a sulfhydryl group for activity. aminopeptidases both metallo-proteinases; posseses broad specifity B-chain of oxidized insulin, other only narrow can split N-terminal basic amino acids peptides.
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