Differential effects of Roux-en-Y gastric bypass surgery on brown and beige adipose tissue thermogenesis

摘要: Abstract Background There are numerous reports of increased energy expenditure after Roux-en-Y gastric bypass (RYGB) surgery in humans and rodent models but the underlying mechanisms remain poorly understood. In present study we assessed at gene expression level whether RYGB leads to recruitment brown adipose tissue (BAT) and/or beige (BeAT) as a means enhanced facultative thermogenesis surgery. Methods Diet-induced obese male Wistar rats were randomized into RYGB-operated (n = 10), sham-operated ad libitum fed (Sham) (n = 7) or body weight matched (BWM) groups (n = 7). At stage postoperatively stabilized reduction, BAT (interscapular), subcutaneous (inguinal) visceral (epididymal perirenal) white (WAT) depots collected fasted state. Expression thermoregulatory genes (UCP1, CIDEA PRDM16) WAT well specific markers BeAT (Ear2 TMEM26) was analyzed using RT-qPCR. Results Compared Sham rats, UCP1 mRNA significantly reduced BWM, not rats. No differences found for proteins between groups. Conclusion The compensatory decrease thermogenic typically associated with loss is attenuated which, opposed BeAT, may contribute overall increases maintenance