Developmental stages and molecular phylogeny of Hepatozoon tuatarae, a parasite infecting the New Zealand tuatara, Sphenodon punctatus and the tick, Amblyomma sphenodonti

作者: James D.K. Herbert , Stephanie S. Godfrey , C. Michael Bull , R. Ian Menz

DOI: 10.1016/J.IJPARA.2010.03.018

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摘要: Abstract The developmental stages of Hepatozoon tuatarae were elucidated in both the tuatara host, Sphenodon punctatus and tick, Amblyomma sphenodonti. PCR amplicons from A. sphenodonti samples identified DNA matching H. tuatarae. Dissection tick showed oogenesis sporogony occurring haemocoel with average mature oocyst size being 236 × 228 μm. Partial sequence data parasite’s small subunit ribosomal gene, obtained by PCR, was used for phylogenetic comparison. Characterisation lifecycle will help conservation management tuatara.

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