No shot in the dark: myxozoans chemically detect fresh fish.

摘要: This work reports the discovery of an hitherto unknown chemical recognition trait enabling a parasitic life cycle in aquatic habitats. We believe this is first record natural, host-derived molecule identified as cue for phylum Myxozoa. The actinospores these parasites attach to fish hosts via polar filaments that are extruded upon mechanical stimulation after preceding trigger contained surface mucus. Our goal was identify signal. separated compounds from purified active fraction derived trout mucus by novel HPLC method. By subsequent nuclear magnetic resonance analysis distinct components and testing bioassays we elicited filament discharge sporoplasm emission three myxozoan spp., Myxobolus cerebralis, pseudodispar Henneguya nuesslini, free nucleosides inosine, 2'-deoxyinosine guanosine. These also activated emission. Nucleosides appear be appropriate cues rapid host waterborne parasite stages since they continuously released into mechanism not specific susceptible species, at least spp. examined. In addition, function nucleobase derivatives semiochemicals uncovered wider impact class systems ecology predicted. relevance disease prevention cell culturing remains explored.